AMBIENTUM BIOETHICA BIOLOGIA CHEMIA DIGITALIA DRAMATICA EDUCATIO ARTIS GYMNAST. ENGINEERING EPHEMERIDES EUROPAEA GEOGRAPHIA GEOLOGIA HISTORIA HISTORIA ARTIUM INFORMATICA IURISPRUDENTIA MATHEMATICA MUSICA NEGOTIA OECONOMICA PHILOLOGIA PHILOSOPHIA PHYSICA POLITICA PSYCHOLOGIA-PAEDAGOGIA SOCIOLOGIA THEOLOGIA CATHOLICA THEOLOGIA CATHOLICA LATIN THEOLOGIA GR.-CATH. VARAD THEOLOGIA ORTHODOXA THEOLOGIA REF. TRANSYLVAN
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Authors: SIMONA TACHE.
Background. Hypothermia and immobilization are commonly used methods in laboratory stress testing. Aims. The study evaluated the effects of chronic hypothermic (5° C) and restraint stress in liver and muscle oxidant/ antioxidant balance, on rats with and without carnitine supplementation. Material and methods. The study was made on four groups of male rats, adult (n = 10 animals / group) Wistar breed, for 15 days): group I- exposed to hypothermic stress (5° C), group II-exposed to anakinetic stress, group III- exposed to combined stress (hypothermic - 5° C - and anakinetic stress), group IV- supplemented with carnitine and exposed to combined stress. Tissues samples (liver and muscle) were used to determine the level and activity of the oxidative stress (OS) indicators - malondialdehyde (MDA), carbonilated proteins (CP) and antioxidant (AO) system –hydrogen donors capacity (HD), thiol groups (SH), reduced gluthatione (GSH). Results. The statistical analysis, performed on the 4 groups, revealed that chronic combined stress induced significant increases for liver and muscle MDA, and decreases for liver SH and muscle GSH, as compared to chronic hypothermic stress. Regarding chronic combined stress, there were significant increases for MDA, and decreases for GSH, in liver and muscle, as compared to chronic anakinetic stress. Carnitine supplementation in chronic combined stress conditions (group IV) induced significant changes by diminishing the liver and muscle MDA levels; liver PC levels, and increasing the DH and SH liver and muscle groups, muscle GSH groups as compared to combined stress group (III). Conclusions. Our experimental results show that chronic combined stress (hypothermic and anakinetic stress) increases the oxidative stress (OS) indicators and decreases those of antioxidant (AO) defense in the studied tissues, as compared to chronic hypothermic and anakinetic stress) Carnitine supplementation in chronic combined stress conditions had benefic effects by diminishing the OS indicators and by increasing the AO defense, in liver and muscle tissue.
Keywords: chronic combined stress, hypothermic stress, anakinetic stress, carnitine, muscle, liver, oxidants/antioxidants balance
